Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury

Elena Iurova; Eugenia Rastorgueva; Evgenii Beloborodov; Evgeniya Pogodina; Aleksandr Fomin; Dmitrii Sugak; Denis Viktorov; Ivan Tumozov; Yury Saenko

doi:10.3390/ph16091314

Pharmaceuticals (Sep 2023)

Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia–Reperfusion Injury

Elena Iurova,
Eugenia Rastorgueva,
Evgenii Beloborodov,
Evgeniya Pogodina,
Aleksandr Fomin,
Dmitrii Sugak,
Denis Viktorov,
Ivan Tumozov,
Yury Saenko

Affiliations

Elena Iurova: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia
Eugenia Rastorgueva: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia
Evgenii Beloborodov: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia
Evgeniya Pogodina: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia
Aleksandr Fomin: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia
Dmitrii Sugak: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia
Denis Viktorov: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia
Ivan Tumozov: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia
Yury Saenko: Laboratory of Research and Development of Peptide Drugs and Vaccines, S. P. Kapitsa Technological Research Institute, Ulyanovsk State University, 432017 Ulyanovsk, Russia

DOI: https://doi.org/10.3390/ph16091314
Journal volume & issue: Vol. 16, no. 9
p. 1314

Abstract

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Ischemia–reperfusion injury (IRI) is a common phenomenon that develops both from natural causes and during major operations. Many intracellular processes mediated by calcium ions are involved in the development of IRI. Currently, chemical calcium channel blockers are used but they have a number of limitations. In this article, we study the effect of the omega-hexatoxin-Hv1a peptide toxin, an alternative to chemical calcium channel blockers, on the mechanisms of IRI development in epithelial cell culture. The toxin was produced using solid phase peptide synthesis. IRI was caused by deprivation of glucose, serum and oxygen. The data obtained demonstrate that the omega-hexatoxin-Hv1a toxin in nanomolar concentrations is able to prevent the development of apoptosis and necrosis in epithelial cells by reducing the concentration of calcium, sodium and potassium ions, as well as by delaying rapid normalization of the pH level, affecting the mitochondrial potential and oxidative stress. This toxin can be used as an alternative to chemical calcium channel blockers for preventing tissue and organ IRI due to its low-dose requirement and high bioavailability.

Published in Pharmaceuticals

ISSN: 1424-8247 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceuticals/

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