Communications Biology (Jun 2022)
Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation
Abstract
CRISPR/Cas9 editing of a PHB-producing H. bluephagenesis strain is used to delete the redundant synthesis pathways of flagella and EPSs, allowing for enhanced self-flocculation, less carbon and energy requirement for metabolic processes and feasible electrotransformation.