Neurobiology of Disease (Sep 1998)
Altered Hippocampal Kainate-Receptor mRNA Levels in Temporal Lobe Epilepsy Patients
- Gary W. Mathern,
- James K. Pretorius,
- Harley I. Kornblum,
- Delia Mendoza,
- Alana Lozada,
- Joao P. Leite,
- Leila Chimelli,
- Donald E. Born,
- Itzhak Fried,
- Americo C. Sakamoto,
- Joao A. Assirati,
- Warwick J. Peacock,
- George A. Ojemann,
- P.David Adelson
Affiliations
- Gary W. Mathern
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- James K. Pretorius
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Harley I. Kornblum
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Delia Mendoza
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Alana Lozada
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Joao P. Leite
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Leila Chimelli
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Donald E. Born
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Itzhak Fried
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Americo C. Sakamoto
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Joao A. Assirati
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Warwick J. Peacock
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- George A. Ojemann
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- P.David Adelson
- Division of Neurosurgery, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, 90095; Mental Retardation Research Center, University of California, Los Angeles, Los Angeles, California, 90095; The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095; Department of Pathology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurology, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP); Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Pathology–Neuropathology and, University of Washington School of Medicine, Seattle, Washington, 98195; Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195
- Journal volume & issue
-
Vol. 5,
no. 3
pp. 151 – 176
Abstract
This study determined whether hippocampal kainate (KA) receptor mRNA levels were increased or decreased in temporal lobe epilepsy patients compared with nonseizure autopsies. Hippocampal sclerosis (HS;n= 17), nonsclerosis (non-HS;n= 11), and autopsy hippocampi (n= 9) were studied for KA1–2 and GluR5–7 mRNA levels using semiquantitativein situhybridization techniques, along with neuron densities. Compared with autopsy hippocampi, HS and non-HS cases showed decreased GluR5 and GluR6 hybridization densities per CA2 and/or CA3 pyramid. Furthermore, HS patients demonstrated increased KA2 and GluR5 hybridization densities per granule cell compared with autopsy hippocampi. These findings indicate that chronic temporal lobe seizures were associated with differential changes in hippocampal KA1–2 and GluR5–7 hybridization densities that vary by subfield and pathology group. In temporal lobe epilepsy patients, these results support the hypothesis that pyramidal cell GluR5 and GluR6 mRNA levels are decreased as a consequence of seizures, and in HS patients granule cell KA2 and GluR5 mRNA levels are increased in association with aberrant fascia dentata mossy fiber sprouting and/or hippocampal neuronal loss.