Sensitive detection of tumor mutations from blood and its application to immunotherapy prognosis

Shuo Li; Zorawar S. Noor; Weihua Zeng; Mary L. Stackpole; Xiaohui Ni; Yonggang Zhou; Zuyang Yuan; Wing Hung Wong; Vatche G. Agopian; Steven M. Dubinett; Frank Alber; Wenyuan Li; Edward B. Garon; Xianghong Jasmine Zhou

doi:10.1038/s41467-021-24457-2

Nature Communications (Jul 2021)

Sensitive detection of tumor mutations from blood and its application to immunotherapy prognosis

Shuo Li,
Zorawar S. Noor,
Weihua Zeng,
Mary L. Stackpole,
Xiaohui Ni,
Yonggang Zhou,
Zuyang Yuan,
Wing Hung Wong,
Vatche G. Agopian,
Steven M. Dubinett,
Frank Alber,
Wenyuan Li,
Edward B. Garon,
Xianghong Jasmine Zhou

Affiliations

Shuo Li: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
Zorawar S. Noor: Department of Medicine, David Geffen School of Medicine at UCLA
Weihua Zeng: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
Mary L. Stackpole: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
Xiaohui Ni: EarlyDiagnostics Inc
Yonggang Zhou: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
Zuyang Yuan: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
Wing Hung Wong: Department of Statistics, Stanford University
Vatche G. Agopian: Department of Surgery, David Geffen School of Medicine at UCLA
Steven M. Dubinett: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
Frank Alber: Institute for Quantitative & Computational Biosciences, University of California at Los Angeles
Wenyuan Li: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles
Edward B. Garon: Department of Medicine, David Geffen School of Medicine at UCLA
Xianghong Jasmine Zhou: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles

DOI: https://doi.org/10.1038/s41467-021-24457-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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It is possible to call single-nucleotide variant (SNV) in cell-free DNA (cfDNA), but the accuracy of detection is often affected by low tumour cfDNA content. Here, the authors develop a method, cfSNV, and show that it can be used even for medium-coverage whole exome sequencing of cfDNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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