Universitas Scientiarum (Apr 2011)
Producción de ácido giberélico a partir de Gibberella fujikuroi utilizando lodo residual municipal como sustrato
Abstract
Production of gibberellic acid from Gibberella fujikuroi using municipal sewage sludge as a substrate. Objective. To use municipalsewage sludge (LRM) from a wastewater treatment plant located in Toluca, State of Mexico, to grow the fungus Gibberella fujikuroi insubmerged fermentation and to produce gibberellic acid (AG3). Materials and methods. We used Gibberella fujikuroi (CDBB: 268). Toobtain AG3, production was verified using as a substrate the standard culture medium (MCE). Gibberellic acid determination was done withhigh performance liquid chromatography (HPLC) with a Varian 9050.9012 equipment. We obtained 6 samples of sludge from a wastewatertreatment plant in Toluca, State of Mexico, that were then characterized. Finally, both substrates (LRM and MCE) were used in submergedfermentation, and GA3 was obtained by extraction and quantified using HPLC. Results. The LRM characterization showed that the organicmatter content (MO) is of 5.20% (w/v) and the total nitrogen content (NT) is of 0.25% (w/v). Such composition is within the range as asubstrate for the production of AG3 by Gibberella fujikuroi. The fungus was cultivated for 3, 8, 13 and 30 days in sterile sewage sludge witha moisture content of 95.6% (w/v) and in standard culture medium (MCE). Samples were processed and analyzed by high performanceliquid chromatography (HPLC). Production of AG3 in the LRM was of 460.06 mg/L after 30 days in submerged fermentation at pH 4.0,and of 1,014.46 mg/L in the control. Conclusion. The nutrient content of LRM is suitable for the growth of the fungus Gibberella fujikuroiand for the production of GA3 when used as a substrate.
