Study of reparative processes and immune mechanisms in liver tissues and blood plasma of rats against the background of loading with plant extracts and induced oxidative stress

Abstract

The aim of the study was to investigate reparative processes and immune mechanisms in rat liver tissues against the background of loading with aqueous extracts of blackberry and common sawfly and induced oxidative stress. 120 rats were used in the experiment. According to group affiliation animals received aqueous extracts of common and blackberry in a dose of 50 mg/100g of weight, 1.5 ml daily for 30 days, and animals of the control group received distilled water in the same mode. Starting from 30 days of the experiment, rats were injected with CCl4 for 6 days. On the 37th day of the experiment, rats were killed according to ethical standards. The number of sinusoidal cells was counted in liver tissues. The content of cytokines in liver homogenates and blood plasma of rats was determined by enzyme-linked immunosorbent assay. Conclusion. Aqueous extracts of fir and blackberry modulate to different degrees the functional state of sinusoidal cells in early periods of toxic exposure to tetrachloromethane, which contributes to the early resolution of the inflammatory process. Exposure of aqueous extracts of fir and blackberry to sinusoidal cells changes the production of regulatory factors, which compensates the speed of recovery processes after toxic exposure.