Study of the Effects of Bioactive Compounds of Cyanobacterium Desmonostoc alborizicum on Pathogenic Fungi of Wheat

Abstract

Wheat, as one of the most economically important crops, constitutes a major part of the human diet. One of the major challenges in wheat preservation is combating various pests, including fungi, through different pesticides. Due to the toxicity caused in agricultural fields by chemical pesticides, there is a growing inclination towards using biopesticides instead. These biopesticides not only possess antimicrobial properties but also aid in the growth and development of crops. In this context, the bioactive compounds present in cyanobacteria are considered as potential biopesticide candidates. Therefore, the aim of this study is to observe the antifungal effect of bioactive compounds from the cyanobacterium Desmonostoc alborizicum on pathogenic fungi affecting wheat. For this purpose, after culturing the cyanobacterial strain Desmonostoc alborizicum for 14 days, the cyanobacterial extract was applied to wheat plants infected with the fungi Alternaria alternata, Fusarium oxysporum, Aspergillus terreus, and Phytophthora nicotiana var. The activity of antioxidant enzymes and MTT assay on 4T1 cells were then evaluated. The results showed that Aspergillus terreus exhibited the highest resistance, while Fusarium oxysporum showed the highest sensitivity to the cyanobacterial extract of Desmonostoc alborizicum. The activity of the enzymes guaiacol peroxidase, superoxide dismutase, catalase, and glutathione peroxidase in infected plants treated with the cyanobacterial extract significantly decreased (p<0.05), indicating effective stress control and enhanced immune response in these treatments. Therefore, the results of this study suggest that the use of Desmonostoc alborizicum extract can be effective as an antifungal agent in protecting wheat and other agricultural crops.

Keywords

• antifungal activity
• Desmonostoc alborizicum
• wheat
• bioactive compounds