Nature Communications (Nov 2020)
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2
- Tânia F. Custódio,
- Hrishikesh Das,
- Daniel J. Sheward,
- Leo Hanke,
- Samuel Pazicky,
- Joanna Pieprzyk,
- Michèle Sorgenfrei,
- Martin A. Schroer,
- Andrey Yu. Gruzinov,
- Cy M. Jeffries,
- Melissa A. Graewert,
- Dmitri I. Svergun,
- Nikolay Dobrev,
- Kim Remans,
- Markus A. Seeger,
- Gerald M. McInerney,
- Ben Murrell,
- B. Martin Hällberg,
- Christian Löw
Affiliations
- Tânia F. Custódio
- Centre for Structural Systems Biology (CSSB), DESY and European Molecular Biology Laboratory Hamburg
- Hrishikesh Das
- Centre for Structural Systems Biology (CSSB) and Karolinska Institutet VR-RÅC
- Daniel J. Sheward
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Leo Hanke
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Samuel Pazicky
- Centre for Structural Systems Biology (CSSB), DESY and European Molecular Biology Laboratory Hamburg
- Joanna Pieprzyk
- Centre for Structural Systems Biology (CSSB), DESY and European Molecular Biology Laboratory Hamburg
- Michèle Sorgenfrei
- Institute of Medical Microbiology, University of Zurich
- Martin A. Schroer
- European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY)
- Andrey Yu. Gruzinov
- European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY)
- Cy M. Jeffries
- European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY)
- Melissa A. Graewert
- European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY)
- Dmitri I. Svergun
- European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY)
- Nikolay Dobrev
- European Molecular Biology Laboratory (EMBL) Heidelberg, Protein Expression and Purification Core Facility
- Kim Remans
- European Molecular Biology Laboratory (EMBL) Heidelberg, Protein Expression and Purification Core Facility
- Markus A. Seeger
- Institute of Medical Microbiology, University of Zurich
- Gerald M. McInerney
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- Ben Murrell
- Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
- B. Martin Hällberg
- Centre for Structural Systems Biology (CSSB) and Karolinska Institutet VR-RÅC
- Christian Löw
- Centre for Structural Systems Biology (CSSB), DESY and European Molecular Biology Laboratory Hamburg
- DOI
- https://doi.org/10.1038/s41467-020-19204-y
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 11
Abstract
Here, the authors isolate several nanobodies from a synthetic library that bind the receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) and neutralize S pseudotyped viruses. Cryo-EM structure of Spike with one nanobody and further biophysical analysis shows competition with ACE2 binding.
