Shiyan dongwu yu bijiao yixue (Feb 2022)

Expression and Significance of Monocarboxylate Transporters in Cortex of Rats After Exercise-induced Fatigue

  • GAO Chen,
  • WANG Wan,
  • LI Yurong,
  • PEI Wenjuan

DOI
https://doi.org/10.12300/j.issn.1674-5817.2021-053
Journal volume & issue
Vol. 42, no. 1
pp. 42 – 47

Abstract

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ObjectiveTo explore the correlation between the adaptation of the body to exercise-induced fatigue and the expression of monocarboxylate transporters (MCTs) in the cerebral cortex.MethodsA total of 42 SD rats were randomly divided into control group and fatigue group 1 to 6. Firstly, the average exhaustive times in each fatigue group were recorded to determine the trend of exercise-induced fatigue after 1, 3, 5, 7, 9 and 14 days exhaustive load treadmill exercises respectively. Subsequently, the changes of MCT1, MCT2 and MCT4 expression in cerebral motor cortex of rat brain were detected by Western blotting and double-labeling immunofluorescence.ResultsAfter 5 days of exercise, the average exhaustive time of rats in fatigue of group 3 reached the lowest level [(67.00±7.07) min] and it recovered to (89.17±9.45) min after 9 days of exercise, the difference was statistically significant (P<0.05). After that, the exercise ability of the rats remained basically constant. Western blotting analysis showed a small amount of MCT1, MCT2 and MCT4 normal expression in cerebral motor cortex of rats' brain in control group. After 5 days of exercise, the expression levels of MCT2 in fatigue of group 3 were significantly increased by 74.2% compared with the control group (P<0.05). After 7 days of exercise, the expression levels of MCT1 and MCT4 in fatigue of group 4 were significantly increased by 89.5% and 92.0% compared with the control group (P<0.05). After that, MCT1, MCT2, and MCT4 maintained high expression. The results of immunofluorescence were consistent with those of Western blotting.ConclusionThe regulation of cerebral lactic acid metabolism represented by the increased expression of MCTs in the cerebral cortex is related to the adaptation of the body to exercise-induced fatigue, which can be used as a target for exercise-induced fatigue medical intervention.

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