Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

Gilberto A. Santiago; Jesús Vázquez; Sean Courtney; Katia Y. Matías; Lauren E. Andersen; Candimar Colón; Angela E. Butler; Rebecca Roulo; John Bowzard; Julie M. Villanueva; Jorge L. Muñoz-Jordan

doi:10.1038/s41467-018-03772-1

Nature Communications (Apr 2018)

Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

Gilberto A. Santiago,
Jesús Vázquez,
Sean Courtney,
Katia Y. Matías,
Lauren E. Andersen,
Candimar Colón,
Angela E. Butler,
Rebecca Roulo,
John Bowzard,
Julie M. Villanueva,
Jorge L. Muñoz-Jordan

Affiliations

Gilberto A. Santiago: Division of Vector Borne Diseases, Dengue Branch, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Jesús Vázquez: Division of Vector Borne Diseases, Dengue Branch, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Sean Courtney: Division of Preparedness and Emerging Infections, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Katia Y. Matías: Division of Vector Borne Diseases, Dengue Branch, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Lauren E. Andersen: Division of Preparedness and Emerging Infections, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Candimar Colón: Division of Vector Borne Diseases, Dengue Branch, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Angela E. Butler: Division of Preparedness and Emerging Infections, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Rebecca Roulo: Division of Preparedness and Emerging Infections, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
John Bowzard: Division of Preparedness and Emerging Infections, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Julie M. Villanueva: Division of Preparedness and Emerging Infections, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases
Jorge L. Muñoz-Jordan: Division of Vector Borne Diseases, Dengue Branch, Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases

DOI: https://doi.org/10.1038/s41467-018-03772-1
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

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The Trioplex real-time RT-PCR assay was developed for detection of Zika virus infections in areas with dengue and chikungunya transmission. Here, Santiago et al. describe the optimization and clinical performance of the assay, showing high sensitivity for detection and differentiation of the three viruses.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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