Computational approach to evaluate scRNA-seq data quality and gene body coverage with SkewC

Abstract

Summary: SkewC is a single-cell RNA sequencing (scRNA-seq) data quality evaluation tool. The approach is based on determining gene body coverage, and its skewness, as a quality metric for each individual cell. SkewC distinguishes between two types of single cells: typical cells with prototypical gene body coverage profiles and skewed cells with skewed gene body coverage profiles. SkewC can be used on any scRNA-seq data as it is independent from the underlying technology used to generate the data.For complete details on the use and execution of this protocol, please refer to Abugessaisa et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Bioinformatics
• Single Cell
• RNAseq