Frontiers in Immunology (May 2014)

Interleukin-12 (IL-12p70) promotes induction of highly potent Th1-like CD4+CD25+T regulatory cells that inhibit allograft rejection in unmodified recipients.

  • Nirupama Darshan Verma,
  • Nirupama Darshan Verma,
  • Bruce Milne Hall,
  • Karren Michelle Plain,
  • Karren Michelle Plain,
  • Catherine M. Robinson,
  • Rochelle eBoyd,
  • Rochelle eBoyd,
  • Giang eTran,
  • Chuanmin eWang,
  • G. Alex eBishop,
  • Suzanne J. Hodgkinson

DOI
https://doi.org/10.3389/fimmu.2014.00190
Journal volume & issue
Vol. 5

Abstract

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In rat models, CD4+CD25+T regulatory cells (Treg) play a key role in the induction and maintenance of antigen specific transplant tolerance, especially in DA rats with PVG cardiac allografts(1, 2). We have previously described generation of alloantigen specific Treg (Ts1), by culture of naïve natural CD4+CD25+ Treg (nTreg) with specific alloantigen and IL-2 for 4 days. These cells express mRNA for IFN-γ receptor (ifngr) and suppress donor but not third party cardiac allograft rejection mediated by alloreactive CD4+T cells at ratios of We induced highly suppressive Th1-like Treg from naïve nTreg in 7 days by culture with alloantigen, first with rIL-2 then with rIL-12p70. These Th1-like Treg delayed specific-donor allograft rejection demonstrating therapeutic potential

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