A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions

Abstract

Van Lindt, Bratek-Skicki et al. show that at carefully selected pH values, proteins can be kept in solution and their LLPS can then be induced by a jump to native pH. This presents a generic method to study the full kinetic trajectory of LLPS under near native conditions that can be easily controlled, providing a platform for the characterization of physiologically relevant phase-separation behaviour of diverse proteins.