Improving the specificity of nucleic acid detection with endonuclease-actuated degradation

Roger S. Zou; Momcilo Gavrilov; Yang Liu; Dominique Rasoloson; Madison Conte; Justin Hardick; Leo Shen; Siqi Chen; Andrew Pekosz; Geraldine Seydoux; Yukari C. Manabe; Taekjip Ha

doi:10.1038/s42003-022-03242-x

Communications Biology (Mar 2022)

Improving the specificity of nucleic acid detection with endonuclease-actuated degradation

Roger S. Zou,
Momcilo Gavrilov,
Yang Liu,
Dominique Rasoloson,
Madison Conte,
Justin Hardick,
Leo Shen,
Siqi Chen,
Andrew Pekosz,
Geraldine Seydoux,
Yukari C. Manabe,
Taekjip Ha

Affiliations

Roger S. Zou: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Momcilo Gavrilov: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
Yang Liu: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
Dominique Rasoloson: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine
Madison Conte: Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine
Justin Hardick: Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine
Leo Shen: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Siqi Chen: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Andrew Pekosz: Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health
Geraldine Seydoux: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine
Yukari C. Manabe: Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine
Taekjip Ha: Department of Biomedical Engineering, Johns Hopkins University School of Medicine

DOI: https://doi.org/10.1038/s42003-022-03242-x
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 9

Abstract

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A new method, SENTINEL, improves the specificity of nucleic acid target sequence identification via a combination of isothermal amplification and sequence-specific DNA endonucleases. The method can discriminate single base-pair differences and takes less than 1 h to complete.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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