Phenosafranin-Based Colorimetric-Sensing Platform for Nitrite Detection Enabled by Griess Assay

Jingzhou Hou; Huixiang Wu; Xin Shen; Chao Zhang; Changjun Hou; Qiang He; Danqun Huo

doi:10.3390/s20051501

Sensors (Mar 2020)

Phenosafranin-Based Colorimetric-Sensing Platform for Nitrite Detection Enabled by Griess Assay

Jingzhou Hou,
Huixiang Wu,
Xin Shen,
Chao Zhang,
Changjun Hou,
Qiang He,
Danqun Huo

Affiliations

Jingzhou Hou: Key Laboratory of Eco-Environment of Three Gorges Region of Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400045, China
Huixiang Wu: Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, China
Xin Shen: Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, China
Chao Zhang: Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin 644000, China
Changjun Hou: Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, China
Qiang He: Key Laboratory of Eco-Environment of Three Gorges Region of Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400045, China
Danqun Huo: Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, China

DOI: https://doi.org/10.3390/s20051501
Journal volume & issue: Vol. 20, no. 5
p. 1501

Abstract

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A facile and effective colorimetric-sensing platform based on the diazotization of phenosafranin for the detection of NO 2 − under acidic conditions using the Griess assay is presented. Diazotization of commercial phenosafranin produces a color change from purplish to blue, which enables colorimetric quantitative detection of NO 2 − . Optimal detection conditions were obtained at a phenosafranin concentration of 0.25 mM, HCl concentration of 0.4 M, and reaction time of 20 min. Under the optimized detection conditions, an excellent linearity range from 0 to 20 μM was obtained with a detection limit of 0.22 μM. Favorable reproducibility and selectivity of the colorimetric sensing platform toward NO 2 − were also verified. In addition, testing spiked ham sausage, bacon, and sprouts samples demonstrated its excellent practicability. The presented colorimetric sensing platform is a promising candidate for the detection of NO 2 − in real applications.

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

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