Frontiers in Nutrition (Jul 2022)

A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury

  • Peng He,
  • Mi Zhang,
  • Meng Zhao,
  • Mengyao Zhang,
  • Benxu Ma,
  • Hongyu Lv,
  • Yantao Han,
  • Dingtao Wu,
  • Zhangfeng Zhong,
  • Wenwen Zhao,
  • Wenwen Zhao

DOI
https://doi.org/10.3389/fnut.2022.961182
Journal volume & issue
Vol. 9

Abstract

Read online

We isolated and purified a novel polysaccharide from the root of Chuanminshen violaceum, namely, Chuanminshen violaceumis polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, in vivo experiment, CVP’s protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe2+ level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis.

Keywords