Nature Communications (Apr 2020)
Synthetic biology based construction of biological activity-related library of fungal decalin-containing diterpenoid pyrones
- Kento Tsukada,
- Shono Shinki,
- Akiho Kaneko,
- Kazuma Murakami,
- Kazuhiro Irie,
- Masatoshi Murai,
- Hideto Miyoshi,
- Shingo Dan,
- Kumi Kawaji,
- Hironori Hayashi,
- Eiichi N. Kodama,
- Aki Hori,
- Emil Salim,
- Takayuki Kuraishi,
- Naoya Hirata,
- Yasunari Kanda,
- Teigo Asai
Affiliations
- Kento Tsukada
- Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku
- Shono Shinki
- Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku
- Akiho Kaneko
- Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku
- Kazuma Murakami
- Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
- Kazuhiro Irie
- Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
- Masatoshi Murai
- Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
- Hideto Miyoshi
- Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
- Shingo Dan
- Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku
- Kumi Kawaji
- Department of Infectious Diseases, International Research Institute of Disaster Science, Tohoku Medical Megabank Organization, Tohoku University, 2-1, Seiryocho, Aoba-ku
- Hironori Hayashi
- Department of Intelligent Network for Infection Control, Graduate School of Medicine, Tohoku University, 2-1, Seiryocho, Aoba-ku
- Eiichi N. Kodama
- Department of Infectious Diseases, International Research Institute of Disaster Science, Tohoku Medical Megabank Organization, Tohoku University, 2-1, Seiryocho, Aoba-ku
- Aki Hori
- Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
- Emil Salim
- Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
- Takayuki Kuraishi
- Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
- Naoya Hirata
- Division of Pharmacology, National Institute of Health Sciences, Tonomachi, Kawasaki-ku
- Yasunari Kanda
- Division of Pharmacology, National Institute of Health Sciences, Tonomachi, Kawasaki-ku
- Teigo Asai
- Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku
- DOI
- https://doi.org/10.1038/s41467-020-15664-4
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 12
Abstract
Combining genome mining and heterologous expression in a genetically tractable host can lead to bioactive natural products discovery and production. Here, the authors employ this strategy for new decalin-containing diterpenoid pyrenes production by expressing native, extended, and shunt pathways in Aspergillus oryzae.
