EFFECT OF CENTRIFUGATION AND ADDITION OF MELATONIN HORMONE TO SEMEN ON CHARACTERISTICS OF SEMINAL PLASMA IN COOLED SEMEN OF IRAQI LOCAL ROOSTERS

Abstract

This experiment conducted in the poultry fields, Department of Animal Production / College of Agriculture, University of Anbar from 1 December 2021 to 2 April 2022 in order to study the effect of semen storage process after centrifugation and replacement of seminal plasma with Lake (1960) diluent to preserve semen with the addition of the hormone melatonin as an antioxidant. This study conducted on 40 roosters of 33 weeks old Iraqi native rooster domestic chickens under standard conditions of temperature, humidity, ventilation and a balanced diet. Semen collecting samples divided into five treatments, three replicates for each one as follows. T1: control, T2: adding diluent as (1:2) semen: diluent only T3: adding a diluent containing the hormone melatonin at a concentration (1 mMol.L‾¹) T4: using Centrifuge with the addition of the dilution in the ratio (1:2) only T5: Use a centrifuge with the addition of a diluent containing the hormone melatonin at a concentration (1 mMol.L‾¹). Results showed that superiority in T3 treatment for seminal plasma traits, as it decreased significantly (P<0.01) in the percentage of protein concentration in plasma for T3 with several storages for 72 hours. significant decrease (P<0.01) in GOT enzyme activity within the T3 treatment compared with treatments, While the rest of the treatment did not record any significant differences. We conclude from this that the addition of the melatonin hormone at a concentration of (1mMol.L‾¹) to the preservation diluents led to an improvement in the characteristics of the seminal plasma.

Keywords

• seminal plasma
• centrifugation
• melatonin hormone
• cryopreservation
• local chicken