Nature Communications (Oct 2019)
Framework engineering to produce dominant T cell receptors with enhanced antigen-specific function
- Sharyn Thomas,
- Fiyaz Mohammed,
- Rogier M. Reijmers,
- Annemarie Woolston,
- Theresa Stauss,
- Alan Kennedy,
- David Stirling,
- Angelika Holler,
- Louisa Green,
- David Jones,
- Katherine K. Matthews,
- David A. Price,
- Benjamin M. Chain,
- Mirjam H. M. Heemskerk,
- Emma C. Morris,
- Benjamin E. Willcox,
- Hans J. Stauss
Affiliations
- Sharyn Thomas
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- Fiyaz Mohammed
- Cancer Immunology and Immunotherapy Centre, Institute for Immunology and Immunotherapy, University of Birmingham, Edgbaston
- Rogier M. Reijmers
- Department of Hematology, Leiden University Medical Center
- Annemarie Woolston
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- Theresa Stauss
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- Alan Kennedy
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- David Stirling
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- Angelika Holler
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- Louisa Green
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- David Jones
- Department of Computer Science, University College London
- Katherine K. Matthews
- Division of Infection and Immunity, Cardiff University School of Medicine
- David A. Price
- Division of Infection and Immunity, Cardiff University School of Medicine
- Benjamin M. Chain
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- Mirjam H. M. Heemskerk
- Department of Hematology, Leiden University Medical Center
- Emma C. Morris
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- Benjamin E. Willcox
- Cancer Immunology and Immunotherapy Centre, Institute for Immunology and Immunotherapy, University of Birmingham, Edgbaston
- Hans J. Stauss
- Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, Royal Free Hospital
- DOI
- https://doi.org/10.1038/s41467-019-12441-w
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 15
Abstract
Increasing TCR cell surface expression can potentiate T cell responses to low-concentrations of antigen. Here the authors identify aminoacids in human TCR variable domains that impact its surface expression, and demonstrate how editing these residues can improve T cell activation and effector function without altering antigen specificity.
