Phenotypical Evaluation of Multi-Drug Resistant Acinetobacter Baumannii

Abstract

Background & Objective: Resistance patterns among nosocomial bacterial pathogens in hospitals may vary widely from country to country at any given point and within the same country over time. Acinetobacter baumannii is one of the most important bacteria that cause hospital-acquired infections. Therefore, surveillance of the antibiotic resistance of Acinetobacter baumannii is necessary, especially in our country, where there is death of relevant data. Materials & Methods: This cross-sectional-descriptive study was performed on 63 MDR isolates of acinetobacter baumannii isolated from patients in Fars province hospitals. After acinetobacter baumannii diagnosis with bacteriological standard tests , we determined their susceptibility to the following antibiotics via the antibiogram method (disk diffusion): cefoxitine; polymixin B; nalidixic acid; meropenem; tobramycin; imipenem; trimethoprim sulphametoxazole; ampicilin-sulbactam; azteronam; rifampicin; ceftazidim; gentamycin; cefotaxim; piperacillin; ciprofloxacin; streptomycin; amikacin; and polymixin B. Minimum inhibitory concentration (MIC ( for imipenem and ciprofloxacin against acinetobacter baumannii isolates was determined using the serial dilution method according to the CLSI guideline. Results: The percentage of acinetobacter baumannii isolates that were resistant to ciprofloxacin, imipenem, and meropenem in the serial dilution method was 77.7%, 40.9%, and 60%, respectively. Conclusion: High antimicrobial resistance in acinetobacter baumannii species was observed in the present study; therefore, it is necessary to implement some approaches for the prevention of bacterial spread.

Keywords

• Acinetobacter
• Antibiotic resistance
• MIC
• Imipenem
• Ciprofloxacin
• Meropenem.