Circular RNA PVT1 acts as a competing endogenous RNA for miR-497 in promoting non-small cell lung cancer progression

Si Qin; Yue Zhao; Gwanyong Lim; Hongjing Lin; Xueli Zhang; Xiaohong Zhang

Biomedicine & Pharmacotherapy (Mar 2019)

Circular RNA PVT1 acts as a competing endogenous RNA for miR-497 in promoting non-small cell lung cancer progression

Si Qin,
Yue Zhao,
Gwanyong Lim,
Hongjing Lin,
Xueli Zhang,
Xiaohong Zhang

Affiliations

Si Qin: Department of Respiration, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin Province, China
Yue Zhao: Department of Respiration, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin Province, China
Gwanyong Lim: Shenzhen College of International Education, First HuangGang Park Street, Shenzhen 518048, Guangdong Province, China
Hongjing Lin: Department of Respiration, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin Province, China
Xueli Zhang: Department of Respiration, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin Province, China
Xiaohong Zhang: Department of Respiration, The First Hospital of Jilin University, No. 71 Xinmin Street, Changchun, 130021, Jilin Province, China; Corresponding author.

Journal volume & issue: Vol. 111
pp. 244 – 250

Abstract

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Circular RNAs (circRNAs) are a class of covalently closed non-coding RNAs and play crucial regulatory roles in human cancer biology. The purpose of the present study was to explore the expression pattern and biological role of circular RNA PVT1 (circPVT1) in non-small cell lung cancer (NSCLC). We firstly found that circPVT1 was overexpressed in clinical NSCLC tissues and cell lines. NSCLC patients with high expression of circPVT1 exhibited aggressive clinicopathological characteristics and poor prognosis. In vitro assays of the NSCLC cell lines (H1299 and A549 cells) demonstrated that knockdown of circPVT1 inhibited NSCLC cell proliferation and induced NSCLC cell apoptosis. We further found that circPVT1 served as a competing endogenous RNA of miR-497 and indirectly regulated Bcl-2 expression in NSCLC cells. Finally, inhibition of miR-497 abrogated the effects of circPVT1 knockdown in NSCLC cells. Taken together, the results from our study indicated circPVT1 acts as an oncogene in NSCLC, and may serve as a promising therapeutic target for NSCLC patients.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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