Amygdalin Promotes Fracture Healing through TGF-β/Smad Signaling in Mesenchymal Stem Cells

Jun Ying; Qinwen Ge; Songfeng Hu; Cheng Luo; Fengyi Lu; Yikang Yu; Taotao Xu; Shuaijie Lv; Lei Zhang; Jie Shen; Di Chen; Peijian Tong; Luwei Xiao; Ju Li; Hongting Jin; Pinger Wang

doi:10.1155/2020/8811963

Stem Cells International (Jan 2020)

Amygdalin Promotes Fracture Healing through TGF-β/Smad Signaling in Mesenchymal Stem Cells

Jun Ying,
Qinwen Ge,
Songfeng Hu,
Cheng Luo,
Fengyi Lu,
Yikang Yu,
Taotao Xu,
Shuaijie Lv,
Lei Zhang,
Jie Shen,
Di Chen,
Peijian Tong,
Luwei Xiao,
Ju Li,
Hongting Jin,
Pinger Wang

Affiliations

Jun Ying: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006 Zhejiang Province, China
Qinwen Ge: First Clinical College of Zhejiang Chinese Medical University, Hangzhou, 310053 Zhejiang Province, China
Songfeng Hu: Department of Orthopaedics, Shaoxing Hospital of Traditional Chinese Medicine, Affiliated with Zhejiang Chinese Medical University, Shaoxing, 312000 Zhejiang Province, China
Cheng Luo: Department of Orthopaedic Surgery, Fuyang Orthopaedics and Traumatology Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
Fengyi Lu: First Clinical College of Zhejiang Chinese Medical University, Hangzhou, 310053 Zhejiang Province, China
Yikang Yu: First Clinical College of Zhejiang Chinese Medical University, Hangzhou, 310053 Zhejiang Province, China
Taotao Xu: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006 Zhejiang Province, China
Shuaijie Lv: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006 Zhejiang Province, China
Lei Zhang: Department of Orthopedics, Xiaoshan District Hospital of Traditional Chinese Medicine of Hangzhou, Hangzhou, 311201 Zhejiang Province, China
Jie Shen: Department of Orthopaedic Surgery, School of Medicine, Washington University, St. Louis, MO 63110, USA
Di Chen: Research Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
Peijian Tong: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006 Zhejiang Province, China
Luwei Xiao: Institute of Orthopaedics and Traumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310053 Zhejiang Province, China
Ju Li: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006 Zhejiang Province, China
Hongting Jin: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006 Zhejiang Province, China
Pinger Wang: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006 Zhejiang Province, China

DOI: https://doi.org/10.1155/2020/8811963
Journal volume & issue: Vol. 2020

Abstract

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Chondrogenesis and subsequent osteogenesis of mesenchymal stem cells (MSCs) and angiogenesis at injured sites are crucial for bone fracture healing. Amygdalin, a cyanogenic glycoside compound derived from bitter apricot kernel, has been reported to inhibit IL-1β-induced chondrocyte degeneration and to stimulate blood circulation, suggesting a promising role of amygdalin in fracture healing. In this study, tibial fractures in C57BL/6 mice were treated with amygdalin. Fracture calluses were then harvested and subjected to radiographic, histological, and biomechanical testing, as well as angiography and gene expression analyses to evaluate fracture healing. The results showed that amygdalin treatment promoted bone fracture healing. Further experiments using MSC-specific transforming growth factor- (TGF-) β receptor 2 conditional knockout (KO) mice (Tgfbr2Gli1-Cre) and C3H10 T1/2 murine mesenchymal progenitor cells showed that this effect was mediated through TGF-β/Smad signaling. We conclude that amygdalin could be used as an alternative treatment for bone fractures.

Published in Stem Cells International

ISSN: 1687-966X (Print); 1687-9678 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine
Website: https://onlinelibrary.wiley.com/journal/4162

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