Possible Mechanism of Sucrose and Trehalose-6-Phosphate in Regulating the Secondary Flower on the Strong Upright Spring Shoots of Blueberry Planted in Greenhouse

Hui-Ling Wu; Sui-Lin Zhang; Xin Feng; Ya-Qian Zhang; Bing-Jie Zhou; Man Cao; Ya-Ping Wang; Bao-Shi Guo; Zhi-Xia Hou

doi:10.3390/plants13172350

Plants (Aug 2024)

Possible Mechanism of Sucrose and Trehalose-6-Phosphate in Regulating the Secondary Flower on the Strong Upright Spring Shoots of Blueberry Planted in Greenhouse

Hui-Ling Wu,
Sui-Lin Zhang,
Xin Feng,
Ya-Qian Zhang,
Bing-Jie Zhou,
Man Cao,
Ya-Ping Wang,
Bao-Shi Guo,
Zhi-Xia Hou

Affiliations

Hui-Ling Wu: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China
Sui-Lin Zhang: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China
Xin Feng: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China
Ya-Qian Zhang: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China
Bing-Jie Zhou: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China
Man Cao: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China
Ya-Ping Wang: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China
Bao-Shi Guo: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China
Zhi-Xia Hou: State Key Laboratory of Efficient Production of Forest Resources, Key Laboratory for Silviculture and Conservation of Ministry of Education, Blueberry Research & Development Center, Beijing Forestry University, Beijing 100083, China

DOI: https://doi.org/10.3390/plants13172350
Journal volume & issue: Vol. 13, no. 17
p. 2350

Abstract

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Secondary flowering is the phenomenon in which a tree blooms twice or more times a year. Along with the development of blueberry (Vaccinium corymbosum L.) fruits in spring, a large number of secondary flowers on the strong upright spring shoots were noticed in blueberries planted in the greenhouse. To reveal the cause and possible regulatory mechanism of the phenomenon, we clarified the phenological characteristics of flower bud differentiation and development on the spring shoots by combining phenological phenotype with anatomical observation. Furthermore, the changes in carbohydrates, trehalose-6-phosphate (Tre6P), and the relationship among the key enzyme regulatory genes for Tre6P metabolism and the key regulatory genes for flower formation during the differentiation process of apical buds and axillary buds were investigated. The results showed that the process of flower bud differentiation and flowering of apical and axillary buds was consistent, accompanied by a large amount of carbohydrate consumption. This process was positively correlated with the expression trends of VcTPS1/2, VcSnRK1, VcFT, VcLFY2, VcSPL43, VcAP1, and VcDAM in general, and negatively correlated with that of VcTPP. In addition, there is a certain difference in the differentiation progress of flower buds between the apical and axillary buds. Compared with axillary buds, apical buds had higher contents of sucrose, fructose, glucose, Tre6P, and higher expression levels of VcTPS2, VcFT, VcSPL43, and VcAP1. Moreover, VcTPS1 and VcTPS2 were more closely related to the physiological substances (sucrose and Tre6P) in axillary bud and apical bud differentiation, respectively. It was suggested that sucrose and trehalose-6-phosphate play a crucial role in promoting flower bud differentiation in strong upright spring shoots, and VcTPS1 and VcTPS2 might play a central role in these activities. Our study provided substantial sight for further study on the mechanism of multiple flowering of blueberries and laid a foundation for the regulation and utilization of the phenomenon of multiple flowering in a growing season of perennial woody plants.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

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