Nature Communications (Jul 2021)
Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress
- Luiza Mendonça,
- Andrew Howe,
- James B. Gilchrist,
- Yuewen Sheng,
- Dapeng Sun,
- Michael L. Knight,
- Laura C. Zanetti-Domingues,
- Benji Bateman,
- Anna-Sophia Krebs,
- Long Chen,
- Julika Radecke,
- Vivian D. Li,
- Tao Ni,
- Ilias Kounatidis,
- Mohamed A. Koronfel,
- Marta Szynkiewicz,
- Maria Harkiolaki,
- Marisa L. Martin-Fernandez,
- William James,
- Peijun Zhang
Affiliations
- Luiza Mendonça
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- Andrew Howe
- Diamond Light Source, Harwell Science and Innovation Campus
- James B. Gilchrist
- Diamond Light Source, Harwell Science and Innovation Campus
- Yuewen Sheng
- Diamond Light Source, Harwell Science and Innovation Campus
- Dapeng Sun
- Department of Structural Biology, University of Pittsburgh, Pittsburgh
- Michael L. Knight
- Sir William Dunn School of Pathology, University of Oxford
- Laura C. Zanetti-Domingues
- Central Laser Facility, Science and Technology Facilities Council, Rutherford Appleton Laboratory
- Benji Bateman
- Central Laser Facility, Science and Technology Facilities Council, Rutherford Appleton Laboratory
- Anna-Sophia Krebs
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- Long Chen
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- Julika Radecke
- Diamond Light Source, Harwell Science and Innovation Campus
- Vivian D. Li
- Murray Edwards College, University of Cambridge
- Tao Ni
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- Ilias Kounatidis
- Diamond Light Source, Harwell Science and Innovation Campus
- Mohamed A. Koronfel
- Diamond Light Source, Harwell Science and Innovation Campus
- Marta Szynkiewicz
- Central Laser Facility, Science and Technology Facilities Council, Rutherford Appleton Laboratory
- Maria Harkiolaki
- Diamond Light Source, Harwell Science and Innovation Campus
- Marisa L. Martin-Fernandez
- Central Laser Facility, Science and Technology Facilities Council, Rutherford Appleton Laboratory
- William James
- Sir William Dunn School of Pathology, University of Oxford
- Peijun Zhang
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- DOI
- https://doi.org/10.1038/s41467-021-24887-y
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 10
Abstract
In this study, Peijun Zhang and colleagues use cryoFIB/SEM volume imaging and soft x-ray cryo-tomography with cryo-electron tomography (cryoET) of cellular periphery, lamellae, and subtomogram averaging to place critical structural events in the SARS-CoV-2 infection cycle in the context of whole-cell images.
