Genome-wide deposition of 6-methyladenine in human DNA reduces the viability of HEK293 cells and directly influences gene expression

Affiliations

Julian Broche: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart
Anja R. Köhler: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart
Fiona Kühnel: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart
Bernd Osteresch: Institute of Biochemistry and Technical Biochemistry, Department of Food Chemistry, University of Stuttgart
Thyagarajan T. Chandrasekaran: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart
Sabrina Adam: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart
Jens Brockmeyer: Institute of Biochemistry and Technical Biochemistry, Department of Food Chemistry, University of Stuttgart
Albert Jeltsch: Institute of Biochemistry and Technical Biochemistry, Department of Biochemistry, University of Stuttgart

Abstract

Global introduction of m6dA into human DNA in HEK293 cells reduces their viability and directly alters their gene expression.