Organic-inorganic nHA-Gelatin/Alginate high strength macroporous cryogel promotes bone regeneration

Wenxuan Shi; Zhuoyuan Li; Lingjie Peng; Xiao Wang; Fei Zheng; Tingshu Su; Qingfeng Huang; Lingyan Cao; Ao Zheng

Smart Materials in Medicine (Sep 2024)

Organic-inorganic nHA-Gelatin/Alginate high strength macroporous cryogel promotes bone regeneration

Wenxuan Shi,
Zhuoyuan Li,
Lingjie Peng,
Xiao Wang,
Fei Zheng,
Tingshu Su,
Qingfeng Huang,
Lingyan Cao,
Ao Zheng

Affiliations

Wenxuan Shi: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China; School of Dental Medicine, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH, 44106, USA
Zhuoyuan Li: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, 639 Zhizaoju Road, Shanghai, 200011, China
Lingjie Peng: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China
Xiao Wang: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China
Fei Zheng: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China
Tingshu Su: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China
Qingfeng Huang: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China; Corresponding author.
Lingyan Cao: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China; Corresponding author.
Ao Zheng: Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai, 200011, China; Corresponding author.

Journal volume & issue: Vol. 5, no. 3
pp. 337 – 347

Abstract

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Macroporous cryogel has the advantages of nutrient exchange and cell growth, and is an ideal material for tissue regeneration. In order to strengthen the machenical properties of cryogel for the widely use, a high strength gelatin/sodium alginate/nano hydroxyapatite (nHA) porous cryogel (GA-HA cryogel) was prepared by a simple freeze-thaw process. The mechanical strength of GA-HA cryogel increased significantly with the increase of nHA content. In vitro studies showed that GA-HA cryogel had good biocompatibility and no obvious cytotoxicity to MC3T3-E1 cells. The results of alkaline phosphatase activity assay and osteocalcin immunofluorescence staining showed that GA-HA1 porous hydrogel system could significantly increase the expression of MC3T3-E1 alkaline phosphatase and osteocalcin when the content of nHA was 1 %. In addition, porous GA-HA cryogel showed good performance in promoting bone regeneration in rat skull defect model. Therefore, the high-strength double network cryogel prepared in this study can provide new applications in bone repair and tissue regeneration.

Published in Smart Materials in Medicine

ISSN: 2590-1834 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology
Website: https://www.keaipublishing.com/en/journals/smart-materials-in-medicine/

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