Microsatellite-based genetic variability and differentiation of hatchery and feral common carp Cyprinus carpio L. (Cyprinidae, Cypriniformes) populations in Croatia

Abstract

Common carp production has an important status in Croatian aquaculture. In addition, the sport fishing of common carp in open waters is very popular, but it is often based on stocking from fish farms. Using fifteen microsatellites, 243 individuals from 5 hatchery and 5 feral populations have been analyzed. A total number of 148 alleles were recorded. However, the mean number of alleles per locus was remarkably low. Pairwise FST values (0.026-0.130) were significant (P<0.01), demonstrating differentiation among populations. The Markov chain method test showed that all the populations deviated from HWE (P <0.05). After sequential Bonferroni correction only the Vrana lake was in HWE in all the loci but MFW20. The factors that may result in genetic divergence and significant reduction of the observed heterozygosity are discussed. AMOVA results for 10 populations indicate that the percentage of the variation among populations was 6.26%, which is lower than the variation within populations (91.04%).

Keywords

• Cyprinus carpio
• common carp
• microsatellites
• population genetics
• Croatia