مجله دانشکده پزشکی اصفهان (Mar 2012)
Establishment of Experimental Autoimmune Encephalitis Model by Injection of Different Combination of Homogenized Brain Tissue of Rat with Complete Freund's Adjuvant
Abstract
Background: Experimental autoimmune encephalitis (EAE) is an animal model of multiple sclerosis (MS). Today in Iran for induction of EAE on rats we need to buy specific encephalitogen such as myelin basic protein (MBP), myelin oligodendrocyte glyco protein (MOG), Proteo lipo protein (PLP) from developed countries. It is very essential for us to find a way for induction of EAE which is cheaper and more rapid that make us independent. It is a fundamental technique.because if we want to study MS, we will need animal model. If we get proper result, we can over come many obstacles and difficulties in future for further investigations on MS and its etiology and its pathophysiological mechanism. Methods: In this study after one of rats exsanguinations the brain tissue was put in a saline solution and then was homogenized with a hemogenizer of Silent Crusher S model. It brought a suspension. A constant amount of Complete Freund's adjuvant (CFA) with different amounts of quantity of this prepared suspension then was combined and was injected subcutaneously to a series of similar rats n = 10. In this investigation all were rats and 590± 10 gr and 3 month old. The administration was repeated with a week interval for some of the rats and the results were compared. Also different combinations were administered and the results were compared. Finally neurological deficit was looked for which was our aim. Findings: This study illustrated that supernatant solution was more encephalitogen than Suspension solution, and this was usable in an applicable technique for induction of Neurological paralysis in rats (P < 0.05) but dose not show any optimum combination of supernatant and CFA for that aim. Conclusion: With modifying old method for induction of EAE we could find preferable one rather than high costly methods which are used today. Also this method should be assessed with further investigations on different mice and rats strains and compared with each other and also with further investigations this method should be compared with other methods in literature.
