Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time

Jan Andreas Ruland; Annika Marie Krüger; Kerstin Dörner; Rohan Bhatia; Sabine Wirths; Daniel Poetes; Ulrike Kutay; Jan Peter Siebrasse; Ulrich Kubitscheck

doi:10.1038/s41467-021-26323-7

Nature Communications (Oct 2021)

Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time

Jan Andreas Ruland,
Annika Marie Krüger,
Kerstin Dörner,
Rohan Bhatia,
Sabine Wirths,
Daniel Poetes,
Ulrike Kutay,
Jan Peter Siebrasse,
Ulrich Kubitscheck

Affiliations

Jan Andreas Ruland: Institute of Physical and Theoretical Chemistry, University of Bonn
Annika Marie Krüger: Institute of Physical and Theoretical Chemistry, University of Bonn
Kerstin Dörner: Institute of Biochemistry, ETH Zurich
Rohan Bhatia: Institute of Physical and Theoretical Chemistry, University of Bonn
Sabine Wirths: Institute of Physical and Theoretical Chemistry, University of Bonn
Daniel Poetes: Institute of Physical and Theoretical Chemistry, University of Bonn
Ulrike Kutay: Institute of Biochemistry, ETH Zurich
Jan Peter Siebrasse: Institute of Physical and Theoretical Chemistry, University of Bonn
Ulrich Kubitscheck: Institute of Physical and Theoretical Chemistry, University of Bonn

DOI: https://doi.org/10.1038/s41467-021-26323-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Ribosomal biogenesis is known to require nuclear to cytoplasmic export, but the precise kinetics remain unclear. Here, the authors use super-resolution confocal microscopy and single molecule tracking to visualize export of single pre-60S particles through nuclear pore complexes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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