Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling

Christian Schmerling; Leonard Sewald; Geronimo Heilmann; Frederick Witfeld; Dominik Begerow; Kenneth Jensen; Christopher Bräsen; Farnusch Kaschani; Herman S. Overkleeft; Bettina Siebers; Markus Kaiser

doi:10.1038/s42003-022-04141-x

Communications Biology (Nov 2022)

Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling

Christian Schmerling,
Leonard Sewald,
Geronimo Heilmann,
Frederick Witfeld,
Dominik Begerow,
Kenneth Jensen,
Christopher Bräsen,
Farnusch Kaschani,
Herman S. Overkleeft,
Bettina Siebers,
Markus Kaiser

Affiliations

Christian Schmerling: Molecular Enzyme Technology and Biochemistry (MEB), Environmental Microbiology and Biotechnology (EMB), Centre for Water and Environmental Research (CWE), Faculty of Chemistry, University of Duisburg-Essen
Leonard Sewald: Department of Chemical Biology, ZMB, Faculty of Biology, University of Duisburg-Essen
Geronimo Heilmann: Department of Chemical Biology, ZMB, Faculty of Biology, University of Duisburg-Essen
Frederick Witfeld: Evolution of Plants and Fungi, Ruhr-University Bochum
Dominik Begerow: Evolution of Plants and Fungi, Ruhr-University Bochum
Kenneth Jensen: Novozymes, Biologiens vej 2, 2800 Kgs
Christopher Bräsen: Molecular Enzyme Technology and Biochemistry (MEB), Environmental Microbiology and Biotechnology (EMB), Centre for Water and Environmental Research (CWE), Faculty of Chemistry, University of Duisburg-Essen
Farnusch Kaschani: Department of Chemical Biology, ZMB, Faculty of Biology, University of Duisburg-Essen
Herman S. Overkleeft: Department of Bio-organic Synthesis, Leiden Institute of Chemistry, Leiden University
Bettina Siebers: Molecular Enzyme Technology and Biochemistry (MEB), Environmental Microbiology and Biotechnology (EMB), Centre for Water and Environmental Research (CWE), Faculty of Chemistry, University of Duisburg-Essen
Markus Kaiser: Department of Chemical Biology, ZMB, Faculty of Biology, University of Duisburg-Essen

DOI: https://doi.org/10.1038/s42003-022-04141-x
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 14

Abstract

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Activity-based protein profiling is used to screen lignocellulose-degrading enzymes from the white rot fungus Phanerochaete chrysosporium to identify those specifically active in the presence of wood substrate.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal