Responses of N<sub>2</sub>O Production and Abundances of Associated Microorganisms to Soil Profiles and Water Regime in Two Paddy Soils

Jichao Zuo; Hongqing Hu; Qingling Fu; Jun Zhu; Heng Zheng; Minghao Mo; Anguo Tu

doi:10.3390/agronomy12030743

Agronomy (Mar 2022)

Responses of N<sub>2</sub>O Production and Abundances of Associated Microorganisms to Soil Profiles and Water Regime in Two Paddy Soils

Jichao Zuo,
Hongqing Hu,
Qingling Fu,
Jun Zhu,
Heng Zheng,
Minghao Mo,
Anguo Tu

Affiliations

Jichao Zuo: Jiangxi Provincial Key Laboratory of Soil Erosion and Prevention, Jiangxi Academy of Water Science and Engineering, Nanchang 330029, China
Hongqing Hu: Key Laboratory of Subtropical Agricultural Resource and Environment, Ministry of Agriculture and Rural Affairs, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
Qingling Fu: Key Laboratory of Subtropical Agricultural Resource and Environment, Ministry of Agriculture and Rural Affairs, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
Jun Zhu: Key Laboratory of Subtropical Agricultural Resource and Environment, Ministry of Agriculture and Rural Affairs, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
Heng Zheng: Key Laboratory of Subtropical Agricultural Resource and Environment, Ministry of Agriculture and Rural Affairs, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
Minghao Mo: Jiangxi Provincial Key Laboratory of Soil Erosion and Prevention, Jiangxi Academy of Water Science and Engineering, Nanchang 330029, China
Anguo Tu: Jiangxi Provincial Key Laboratory of Soil Erosion and Prevention, Jiangxi Academy of Water Science and Engineering, Nanchang 330029, China

DOI: https://doi.org/10.3390/agronomy12030743
Journal volume & issue: Vol. 12, no. 3
p. 743

Abstract

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Soil moisture is one of the critical factors affecting N2O emissions. The water regime affects the physical and chemical properties of paddy soil in different soil layers, which, in turn, affects N2O emissions and microbial growth. However, there are few reports on the effects of different soil layers and soil moisture conditions on N2O emission characteristics and microbial mechanisms. A 21-day microcosm experiment was performed to research the effects of soil moisture levels (60%, 100%, and 200% water holding capacity, WHC) and different soil layers (0–10, 10–20, and 20–40 cm) on N2O emissions in hydromorphic and gleyed paddy soils. Function microbes involved in nitrification and denitrification were determined by quantitative PCR. Moreover, the abiotic variables pH, Eh, and exchangeable Fe2+, Fe3+, NH4+-N, and NO3−-N were also analyzed. Results showed that N2O emissions of gleyed paddy soil were significantly higher than that of hydromorphic paddy soil, which was consistent with the result of the abundance of nitrifier and denitrifier in the two paddy soils. Soil depth, water content, and their interaction significantly affected N2O emission (p 2O from each layer of the two paddy soils at 100% and 200% WHC were significantly higher than that under 60% WHC (p 2O emissions decreased significantly with the increase of soil depth (p nirK and nosZ) function genes with soil depth. The abundance of AOB, AOA, and nirK and nosZ genes decreased significantly with soil depth (p 2+ and Fe3+ were positively correlated with N2O emissions and the abundance of AOB, AOA, and nirK and nosZ genes. These results indicate that N2O emissions and the abundance of associated microbes are selectively affected by soil moisture and soil layers in the two paddy soils.

Published in Agronomy

ISSN: 2073-4395 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture
Website: http://www.mdpi.com/journal/agronomy

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