Changes in the Biochemical Parameters of Rat Blood when Administering Toxic Doses of Sodium Valproate

Abstract

Background: The aim of this research was to study the regularities of changes in blood biochemical parameters in outbred rats exposed to the toxic effect of sodium valproate (SV) in the experiment. Methods and Results: The study was performed on 94 white outbred male rats, weighing 190-210g. According to the research protocol, all animals were divided into two groups: Intact Group (IG, n=30) and Experimental Group (EG, n=64). Animals of EG were given the anticonvulsant SV in a toxic dose of 600 mg/kg, intragastrically, once a day, for 7 (n=23), 14 (n=13), 21 (n=13) and 28 (n=15) days. The animals were withdrawn from the experiment after 7, 14, 21 and 28 days using diethyl ether. The following biochemical parameters in blood serum were determined: AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALP (alkaline phosphatase), GGT (γ-glutamyltransferase), TP (total protein), glucose, direct bilirubin, TC (total cholesterol), and TG(triglycerides). The administration of toxic doses of SV to experimental animals was accompanied by changes in the biochemical status of blood, especially pronounced on Days 21 and 28 of the experiment. In particular, the activity of enzymes AST, ALT, ALP, GGT increases, the levels of TP and glucose decrease, and the levels of direct bilirubin, TG and TC, increase. Conclusion: The obtained data showed that the overdose of SV significantly worsens the blood biochemical parameters, which may indicate a toxic damage of hepatocytes. The studied biochemical parameters should be used to assess late effects of anticonvulsants on the body.

Keywords

• rats
• sodium valproate
• toxic dose
• biochemical parameters