Sample preparation methods for N-glycomics

Abstract

Aberrant glycosylation has been associated with the human disease since it involves crucial mechanisms such as tumor onset, development, and progression. This review focuses on the most commonly used sample preparation methods for standard glycoprotein, serum, cell, and tissue samples in N-glycan analysis studies. We aim to provide readers with an overview of N-glycomics sample preparation methods for profiling N-glycans. From this perspective, this review illustrates the main strategies of N-glycan analysis using different techniques. The molecular information obtained from glycomics analysis has allowed new visions into the mechanism concerning aberrant glycosylation.

Keywords

• Glycomics
• Sample preparation
• Glycans
• Glycosylation
• Glycopeptides
• Glycopeptide enrichment