Mouse Satellite Cell Isolation and Transplantation

Elia Angelino; Simone Reano; Michele Ferrara; Emanuela Agosti; Hana Sustova; Valeria Malacarne; Sara Clerici; Andrea Graziani; Nicoletta  Filigheddu

doi:10.21769/BioProtoc.2696

Bio-Protocol (Jan 2018)

Mouse Satellite Cell Isolation and Transplantation

Elia Angelino,
Simone Reano,
Michele Ferrara,
Emanuela Agosti,
Hana Sustova,
Valeria Malacarne,
Sara Clerici,
Andrea Graziani,
Nicoletta Filigheddu

Affiliations

Elia Angelino: Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM), Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Simone Reano: Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM)
Michele Ferrara: Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM), Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Emanuela Agosti: Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM)
Hana Sustova: Department of Translational Medicine, University of Piemonte Orientale, Novara, ItaliaIstituto Interuniversitario di Miologia (IIM)
Valeria Malacarne: Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyDivision of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Sara Clerici: Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Andrea Graziani: Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM), Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy
Nicoletta Filigheddu: Department of Translational Medicine, University of Piemonte Orientale, Novara, ItalyIstituto Interuniversitario di Miologia (IIM)

DOI: https://doi.org/10.21769/BioProtoc.2696
Journal volume & issue: Vol. 8, no. 2

Abstract

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Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs (Reano et al., 2017).

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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