Морфологія (Oct 2020)
Morphological features of the epithelium of the mucous membrane of the bladder of rats after administration of the antigen
Abstract
Background. Diseases of the urinary system and the bladder in particular (15-65% of the general pathology of the genitourinary system (SHS) is an urgent problem of medicine. The causes of these diseases are often associated with intrauterine infection. The effect of antigenic (AH) stimulation (immunomorphological influence) is poorly understood today, and aroused interest in this work. The aim of the study was to study the morphological features of the epithelium of the urinary bladder mucosa in rats during postnatal ontogenesis after intrauterine exposure to antigens. Methods. The study was carried out on 192 Wistar rats. Animals were divided into 3 groups: intact rats, control rats, which were injected with NaCl 0.9%, experimental rats, which were injected with staphylococcal toxoid intrafetally on the 18th day of intrauterine development. In each group, 64 animals were studied in 8 age periods: 1, 7, 14, 21, 30, 45, 60 and 90 days of life were taken into account (8 rats in each age cohort). The study was carried out using histological, morphometric and electron microscopic methods. The maintenance and manipulation of animals was carried out in accordance with the "General ethical principles of animal experiments." Results. When studying the urinary bladder mucosa of rats after exposure to the antigen, it was found that in the first 45 days there is a pronounced disorganization of the epithelium, retention of intracellular fluid, followed by partial restoration of structure by the end of the observation period. The thickness of the epithelium in experimental rats prevails over that in intact rats during most of the study period. It is important that by the end of the observation period (90 days) the epithelium in the experimental group is thinner than in the intact one.
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