Tracking neuronal motility in live murine retinal explants

Nozie D. Aghaizu; Katherine M. Warre-Cornish; Martha R. Robinson; Robin R. Ali; Rachael A. Pearson

STAR Protocols (Dec 2021)

Tracking neuronal motility in live murine retinal explants

Nozie D. Aghaizu,
Katherine M. Warre-Cornish,
Martha R. Robinson,
Robin R. Ali,
Rachael A. Pearson

Affiliations

Nozie D. Aghaizu: University College London, Institute of Ophthalmology, London EC1V 9EL, UK; Corresponding author
Katherine M. Warre-Cornish: University College London, Institute of Ophthalmology, London EC1V 9EL, UK
Martha R. Robinson: University College London, Institute of Ophthalmology, London EC1V 9EL, UK
Robin R. Ali: University College London, Institute of Ophthalmology, London EC1V 9EL, UK
Rachael A. Pearson: University College London, Institute of Ophthalmology, London EC1V 9EL, UK; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 101008

Abstract

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Summary: The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated in silico data handling are provided.For complete details on the use and execution of this protocol, please refer to Aghaizu et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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