Modification of bimetal Zn/ Mg MOF with nanoparticles Fe3O4 and Fe3O4@SiO2, investigation of the peroxidase-like activity of these compounds by calorimetry and fluorimetry methods

Abstract

In this article; the bimetal metal-organic framework Zn/Mg (Zn/Mg MOF) is synthesized.Then Zn/Mg MOF bimetal was combined with Fe3O4 and Fe3O4@SiO2, and composites of Fe3O4@ SiO2/MOF/Dextrin, Fe3O4@SiO2/MOF, Fe3O4@MOF/Dextrin and Fe3O4@MOF made. The peroxidase-like activity of these compounds was investigated and compared by calorimetric Resazurin (Rz) and O-phenylenediamine (OPD); (Rz-H2O2, OPD-H2O2) and fluorimetric Rz and terephtalic acid (TA); (Rz-H2O2, TA-H2O2). The Fe3O4@ MOF/Dextrin composite has the highest peroxidase-like activity. The effect factors (amount of pH (6), the values of TA (1.37 mM), H2O2 (0.025 mM), reaction time (8.15 min), and amount of Composite (116.67 mg)) to increase the catalytic activity of Fe3O4@ MOF/Dextrin measured by chemometrics method. The most suitable linear range of the calibration curve by the TA-H2O2 -Composite fluorimetric method is 1–600 μg L−1, and the detection limit is 2.27 μg L−1. The relative standard deviation (RSD%) for measuring concentration atropine 1 μg L−1 (n = 6) is 1.18%. Finally, from this system for measuring atropine extracted by the Liquid-liquid extraction (LLE) method in two types of plants, D. Innoxia north and west and D. stramonium north and west of Iran (118.25 μg L−1, 79.80 μg L−1) and (18.477 μg L−1, 9.27 μg L−1) used, respectively.

Keywords

• Datura
• Composite
• Peroxidase-like
• Fluorometry
• Calorimetry
• Atropine