Functional Characterization of the Monogalactosyldiacylglycerol Synthase Gene ptMGD2 in the Diatom Phaeodactylum tricornutum

Shuo Shang; Ruyi Liu; Ruyi Liu; Ling Luo; Xitong Li; Shengqiang Zhang; Yi Zhang; Peng Zheng; Peng Zheng; Zhuo Chen; Zhuo Chen; Baoshan Wang

doi:10.3389/fmars.2022.874448

Frontiers in Marine Science (Jul 2022)

Functional Characterization of the Monogalactosyldiacylglycerol Synthase Gene ptMGD2 in the Diatom Phaeodactylum tricornutum

Shuo Shang,
Ruyi Liu,
Ruyi Liu,
Ling Luo,
Xitong Li,
Shengqiang Zhang,
Yi Zhang,
Peng Zheng,
Peng Zheng,
Zhuo Chen,
Zhuo Chen,
Baoshan Wang

Affiliations

Shuo Shang: Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, China
Ruyi Liu: Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, China
Ruyi Liu: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei University, Wuhan, China
Ling Luo: Laboratory of Bacterial Vaccine, Wuhan Institute of Biological Products Co., Ltd., Wuhan, China
Xitong Li: Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, China
Shengqiang Zhang: Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, China
Yi Zhang: Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, China
Peng Zheng: Institute of Biology and Medicine, Wuhan University Science Technology, Wuhan, China
Peng Zheng: College of Life Science and Healthy, Wuhan University Science Technology, Wuhan, China
Zhuo Chen: Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, China
Zhuo Chen: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei University, Wuhan, China
Baoshan Wang: Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, China

DOI: https://doi.org/10.3389/fmars.2022.874448
Journal volume & issue: Vol. 9

Abstract

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Monogalactosyldiacylglycerol (MGDG) is the most abundant polar lipid in thylakoid membrane, wherein it plays critical roles related to thylakoid membrane assembly and function in diatoms. However, diatom MGDG biosynthesis has not been fully characterized. In this study, we investigated the role of a novel MGDG synthase (ptMGD2), which is one of the key enzymes for MGDG biosynthesis, in the model diatom Phaeodactylum tricornutum. An analysis of subcellular localization demonstrated that the ptMGD2 is mainly localized in plastids. Gene disruption by gene editing of ptMGD2 resulted in delayed growth, decrease in oxygen evolution rate, reduced MGDG and digalactosyldiacylglycerol (using MGDG as the substrate) content as well as lipid remodeling. Considered together, these observations provide novel insights into the importance of ptMGD2 for regulating MGDG biosynthesis and its potential roles in biotechnical application of Phaeodactylum.

Published in Frontiers in Marine Science

ISSN: 2296-7745 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Natural history (General): General. Including nature conservation, geographical distribution
Website: https://www.frontiersin.org/journals/marine-science

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