Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics

Abstract

Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences.

Keywords

• Mitochondria
• Protease
• Shotgun
• Top-down
• Electroelution