Pathogens (Jun 2021)

High Predatory Capacity of a Novel <i>Arthrobotrys oligospora</i> Variety on the Ovine Gastrointestinal Nematode <i>Haemonchus contortus</i> (Rhabditomorpha: Trichostrongylidae)

  • Fabián Arroyo-Balán,
  • Fidel Landeros-Jaime,
  • Roberto González-Garduño,
  • Cristiana Cazapal-Monteiro,
  • Maria Sol Arias-Vázquez,
  • Gabriela Aguilar-Tipacamú,
  • Edgardo Ulises Esquivel-Naranjo,
  • Juan Mosqueda

DOI
https://doi.org/10.3390/pathogens10070815
Journal volume & issue
Vol. 10, no. 7
p. 815

Abstract

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With the worldwide development of anthelmintic resistance, new alternative approaches for controlling gastrointestinal nematodes in sheep are urgently required. In this work, we identified and characterized native nematode-trapping fungi. We collected seven isolates of fungi with the capacity to form adhesive, three-dimensional networks as the main mechanism to capture, kill, and consume nematodes. The nematode-trapping fungi were classified into two groups; the first group includes the R2-13 strain, showing faster growth, abundant aerial hyphae, scarce conidia production, bigger conidia, and it formed a clade with Arthrobotrys oligospora sensu stricto. The second comprises the A6, A12, A13, R2-1, R2-6, and R2-14 strains, showing a growth adhering to the culture medium, forming little aerial hyphae, smaller conidia, and these formed a sister clade to A. oligospora. Except for the R2-6 strain, conidia production was induced by light. In all the strains, the predatory capacity against the sheep gastrointestinal nematode Haemonchus contortus was greater than 58% compared with the control group. The A6 and A13 strains were the most active against the infective H. contortus third instar (L3) larvae, with an average capture capacity of 91%. Altogether, our results support evidence for a novel A. oligospora variety with high nematode-trapping activity and promissory in helminthic control.

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