Journal of Clinical Medicine (Aug 2017)
Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
- Sharlaa Badal-Faesen,
- Cynthia Firnhaber,
- Michelle A. Kendall,
- Xingye Wu,
- Beatriz Grinsztejn,
- Rodrigo Otavio da Silva Escada,
- Michel Fernandez,
- Evelyn Hogg,
- Ian Sanne,
- Pamela Johnson,
- David Alland,
- Gerald H. Mazurek,
- Debra A. Benator,
- Anne F. Luetkemeyer,
- the AIDS Clinical Trials Group A5295,
- Tuberculosis Trials Consortium Study 34 Teams
Affiliations
- Sharlaa Badal-Faesen
- Clinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South Africa
- Cynthia Firnhaber
- Clinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South Africa
- Michelle A. Kendall
- Center for Biostatistics in AIDS Research, Harvard T.H. Chan School of Public Health Boston, Boston, MA 02115, USA
- Xingye Wu
- Center for Biostatistics in AIDS Research, Harvard T.H. Chan School of Public Health Boston, Boston, MA 02115, USA
- Beatriz Grinsztejn
- Instituto Nacional de Infectologia Evandro Chagas-INI-Fiocruz, Rio de Janeiro 21.045-900, Brazil
- Rodrigo Otavio da Silva Escada
- Instituto Nacional de Infectologia Evandro Chagas-INI-Fiocruz, Rio de Janeiro 21.045-900, Brazil
- Michel Fernandez
- Pulmonary and Critical Care Department, Baylor Scott and White Hospital, Temple, TX 76508, USA
- Evelyn Hogg
- Social & Scientific Systems, Inc., Silver Spring, MD 20910, USA
- Ian Sanne
- Clinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South Africa
- Pamela Johnson
- Cepheid Inc., Sunnyvale, CA 94089, USA
- David Alland
- Division of Infectious Disease, Rutgers-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA
- Gerald H. Mazurek
- Division of Tuberculosis Elimination, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
- Debra A. Benator
- The Veterans Affairs Medical Center, The George Washington University, Washington, DC 20422, USA
- Anne F. Luetkemeyer
- Division of HIV, Infectious Diseases and Global Medicine, Zuckerberg San Francisco General, University of California, San Francisco, CA 94110, USA
- the AIDS Clinical Trials Group A5295
- Clinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South Africa
- Tuberculosis Trials Consortium Study 34 Teams
- Clinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South Africa
- DOI
- https://doi.org/10.3390/jcm6080078
- Journal volume & issue
-
Vol. 6,
no. 8
p. 78
Abstract
As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8).
Keywords
