Cell Reports Medicine (Jun 2021)
Longitudinal analysis of humoral immunity against SARS-CoV-2 Spike in convalescent individuals up to 8 months post-symptom onset
- Sai Priya Anand,
- Jérémie Prévost,
- Manon Nayrac,
- Guillaume Beaudoin-Bussières,
- Mehdi Benlarbi,
- Romain Gasser,
- Nathalie Brassard,
- Annemarie Laumaea,
- Shang Yu Gong,
- Catherine Bourassa,
- Elsa Brunet-Ratnasingham,
- Halima Medjahed,
- Gabrielle Gendron-Lepage,
- Guillaume Goyette,
- Laurie Gokool,
- Chantal Morrisseau,
- Philippe Bégin,
- Valérie Martel-Laferrière,
- Cécile Tremblay,
- Jonathan Richard,
- Renée Bazin,
- Ralf Duerr,
- Daniel E. Kaufmann,
- Andrés Finzi
Affiliations
- Sai Priya Anand
- Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada; Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Jérémie Prévost
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Manon Nayrac
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Guillaume Beaudoin-Bussières
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Mehdi Benlarbi
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Romain Gasser
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Nathalie Brassard
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Annemarie Laumaea
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Shang Yu Gong
- Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada; Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Catherine Bourassa
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Elsa Brunet-Ratnasingham
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Halima Medjahed
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Gabrielle Gendron-Lepage
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Guillaume Goyette
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Laurie Gokool
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Chantal Morrisseau
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada
- Philippe Bégin
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; CHU Ste-Justine, Montreal, QC H3T 1C5, Canada
- Valérie Martel-Laferrière
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Cécile Tremblay
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Jonathan Richard
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
- Renée Bazin
- Héma-Québec, Affaires Médicales et Innovation, Quebec, QC G1V 5C3, Canada
- Ralf Duerr
- Departments of Pathology and Microbiology, New York University School of Medicine, New York, NY 10016, USA; Corresponding author
- Daniel E. Kaufmann
- Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Médecine, Université de Montréal, Montreal, QC H3T 1J4, Canada; Corresponding author
- Andrés Finzi
- Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada; Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada; Corresponding author
- Journal volume & issue
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Vol. 2,
no. 6
p. 100290
Abstract
Summary: With the recent approval of highly effective coronavirus disease 2019 (COVID-19) vaccines, functional and lasting immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently under investigation as antibody levels in plasma were shown to decline during convalescence. Since the absence of antibodies does not equate to absence of immune memory, we evaluate the presence of SARS-CoV-2-specific memory B cells in convalescent individuals. Here, we report a longitudinal assessment of humoral immune responses on 32 donors up to 8 months post-symptom onset. Our observations indicate that anti-Spike and anti-receptor binding domain (RBD) immunoglobulin M (IgM) in plasma decay rapidly, whereas the reduction of IgG is less prominent. Neutralizing activity also declines rapidly when compared to Fc-effector functions. Concomitantly, the frequencies of RBD-specific IgM+ B cells wane significantly when compared to RBD-specific IgG+ B cells, which remain stable. Our results add to the current understanding of immune memory following SARS-CoV-2 infection, which is critical for secondary infection prevention and vaccine efficacy.
