Potent and Synergistic Extract Combinations from Terminalia Catappa, Terminalia Mantaly and Monodora tenuifolia Against Pathogenic Yeasts

Thierry Kammalac Ngouana; Cedric Derick Jiatsa Mbouna; Rufin Marie Toghueo Kuipou; Marthe Aimée Tchuente Tchuenmogne; Elisabeth Menkem Zeuko’o; Vincent Ngouana; Michèle Mallié; Sebastien Bertout; Fabrice Fekam Boyom

doi:10.3390/medicines2030220

Medicines (Aug 2015)

Potent and Synergistic Extract Combinations from Terminalia Catappa, Terminalia Mantaly and Monodora tenuifolia Against Pathogenic Yeasts

Thierry Kammalac Ngouana,
Cedric Derick Jiatsa Mbouna,
Rufin Marie Toghueo Kuipou,
Marthe Aimée Tchuente Tchuenmogne,
Elisabeth Menkem Zeuko’o,
Vincent Ngouana,
Michèle Mallié,
Sebastien Bertout,
Fabrice Fekam Boyom

Affiliations

Thierry Kammalac Ngouana: Antimicrobial Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Messa-Yaoundé, Cameroon
Cedric Derick Jiatsa Mbouna: Antimicrobial Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Messa-Yaoundé, Cameroon
Rufin Marie Toghueo Kuipou: Antimicrobial Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Messa-Yaoundé, Cameroon
Marthe Aimée Tchuente Tchuenmogne: Laboratory of Natural Products and Organic Synthesis, Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Messa-Yaoundé, Cameroon
Elisabeth Menkem Zeuko’o: Antimicrobial Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Messa-Yaoundé, Cameroon
Vincent Ngouana: Antimicrobial Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Messa-Yaoundé, Cameroon
Michèle Mallié: IRD UMI 233 TransVIHMI—UM INSERM U1175 TransVIHMI Laboratoire de Parasitologie et Mycologie Médicale, UFR Pharmacie, Université de Montpellier 1, 15, Avenue Charles Flahault—BP 14491 34093 Montpellier, France
Sebastien Bertout: IRD UMI 233 TransVIHMI—UM INSERM U1175 TransVIHMI Laboratoire de Parasitologie et Mycologie Médicale, UFR Pharmacie, Université de Montpellier 1, 15, Avenue Charles Flahault—BP 14491 34093 Montpellier, France
Fabrice Fekam Boyom: Antimicrobial Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Messa-Yaoundé, Cameroon

DOI: https://doi.org/10.3390/medicines2030220
Journal volume & issue: Vol. 2, no. 3
pp. 220 – 235

Abstract

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Mycoses caused by Candida and Cryptococcus species, associated with the advent of antifungal drug resistance have emerged as major health problems. Improved control measures and innovative therapies are needed. This paper describes results from the screening of bio-guided fractionated extracts alone and combinations of Terminalia catappa, Terminalia mantaly and Monodora tenuifolia harvested in Cameroon. Crude ethanolic, hydro-ethanolic and aqueous extracts and bio-guided fractions were screened for antifungal activity against isolates of C. albicans, C. glabrata, C. parapsilosis and Cr. neoformans and the reference strain C. albicans NR-29450. Minimal inhibitory concentrations (MIC) were determined using a broth micro dilution method according to the Clinical & Laboratory Standards Institute (CLSI). Time kill kinetics of extracts alone and in combination were also evaluated. Extracts from T. mantaly stem bark were the most active with the best MIC values ranging from 0.04 mg/mL to 0.16 mg/mL. Synergistic interactions were observed with combinations of sub-fractions from M. tenuifolia, T. mantaly and T. catappa. Combination of sub-fractions from M. tenuifolia and T. mantaly (C36/C12) showed synergistic interaction and fungicidal effect against four out of five tested yeasts. These results support further investigation of medicinal plant extracts alone and in combination as starting points for the development of alternative antifungal therapy.

Published in Medicines

ISSN: 2305-6320 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/medicines

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