Real-time high dynamic range laser scanning microscopy

C. Vinegoni; C. Leon Swisher; P. Fumene Feruglio; R. J. Giedt; D. L. Rousso; S. Stapleton; R. Weissleder

doi:10.1038/ncomms11077

Nature Communications (Apr 2016)

Real-time high dynamic range laser scanning microscopy

C. Vinegoni,
C. Leon Swisher,
P. Fumene Feruglio,
R. J. Giedt,
D. L. Rousso,
S. Stapleton,
R. Weissleder

Affiliations

C. Vinegoni: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
C. Leon Swisher: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
P. Fumene Feruglio: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
R. J. Giedt: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
D. L. Rousso: Department of Molecular and Cell Biology, Center for Brain Science, Harvard University
S. Stapleton: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center
R. Weissleder: Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center

DOI: https://doi.org/10.1038/ncomms11077
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 13

Abstract

Read online

Confocal and multiphoton fluorescence microscopy often suffers from low dynamic range. Here the authors develop a high dynamic range, laser scanning fluorescence technique by simultaneously recording different light intensity ranges. The method can be adapted to commercial systems.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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