Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration

Manuel J. Carrasco; Suman Alishetty; Mohamad-Gabriel Alameh; Hooda Said; Lacey Wright; Mikell Paige; Ousamah Soliman; Drew Weissman; Thomas E. Cleveland; Alexander Grishaev; Michael D. Buschmann

doi:10.1038/s42003-021-02441-2

Communications Biology (Aug 2021)

Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration

Manuel J. Carrasco,
Suman Alishetty,
Mohamad-Gabriel Alameh,
Hooda Said,
Lacey Wright,
Mikell Paige,
Ousamah Soliman,
Drew Weissman,
Thomas E. Cleveland,
Alexander Grishaev,
Michael D. Buschmann

Affiliations

Manuel J. Carrasco: Department of Bioengineering, George Mason University
Suman Alishetty: Department of Bioengineering, George Mason University
Mohamad-Gabriel Alameh: Perelman School of Medicine, University of Pennsylvania
Hooda Said: Department of Bioengineering, George Mason University
Lacey Wright: Department of Bioengineering, George Mason University
Mikell Paige: Department of Chemistry & Biochemistry, George Mason University
Ousamah Soliman: Perelman School of Medicine, University of Pennsylvania
Drew Weissman: Perelman School of Medicine, University of Pennsylvania
Thomas E. Cleveland: Institute for Bioscience and Biotechnology Research National Institute of Standards and Technology
Alexander Grishaev: Institute for Bioscience and Biotechnology Research National Institute of Standards and Technology
Michael D. Buschmann: Department of Bioengineering, George Mason University

DOI: https://doi.org/10.1038/s42003-021-02441-2
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 15

Abstract

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Carrasco et al. report a method that can predict the lipid nanoparticles (LNP) pKa from the structure of the ionizable lipid. They investigate the delivery efficiency for intramuscular and intravascular administration and propose design principles to limit off-target systemic distribution and expression for mRNA LNP vaccines.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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