Genome-wide tiled detection of circulating Mycobacterium tuberculosis cell-free DNA using Cas13

Sri Gowtham Thakku; Jackson Lirette; Kanagavel Murugesan; Julie Chen; Grant Theron; Niaz Banaei; Paul C. Blainey; James Gomez; Sharon Y. Wong; Deborah T. Hung

doi:10.1038/s41467-023-37183-8

Nature Communications (Mar 2023)

Genome-wide tiled detection of circulating Mycobacterium tuberculosis cell-free DNA using Cas13

Sri Gowtham Thakku,
Jackson Lirette,
Kanagavel Murugesan,
Julie Chen,
Grant Theron,
Niaz Banaei,
Paul C. Blainey,
James Gomez,
Sharon Y. Wong,
Deborah T. Hung

Affiliations

Sri Gowtham Thakku: Broad Institute of MIT and Harvard
Jackson Lirette: Broad Institute of MIT and Harvard
Kanagavel Murugesan: Department of Pathology, Stanford University School of Medicine
Julie Chen: Broad Institute of MIT and Harvard
Grant Theron: DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research and SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University
Niaz Banaei: Department of Pathology, Stanford University School of Medicine
Paul C. Blainey: Broad Institute of MIT and Harvard
James Gomez: Broad Institute of MIT and Harvard
Sharon Y. Wong: Broad Institute of MIT and Harvard
Deborah T. Hung: Broad Institute of MIT and Harvard

DOI: https://doi.org/10.1038/s41467-023-37183-8
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 13

Abstract

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In this work, the authors developed a multiplexed, minimally invasive, CRISPR-Cas13-based approach to detect Mycobacterium tuberculosis cell-free DNA in the plasma of active pulmonary tuberculosis patients.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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