EFFECT OF COPPER SULPHATE PENTAHYDRATE (CuSO4·5H₂O) AND RHIZOBACTERIA ON THE GERMINATION OF ROSELLE (Hibiscus sabdariffa L.)

Abstract

Background: Heavy metals are perceived as the most persistent and dangerous. Cu is essential for plants, in excess it causes detrimental effects on plants and human health. Plant growth promoting bacteria have been mainly used in agriculture to promote seed germination, plant vigor and growth, and improve crop yields. Objective: To analyse the effect of germination in two roselle varieties that were inoculated with extracellular plant growth-promoting rhizobacteria of the genus Pseudomonas and in seven solutions of copper sulphate pentahydrate. Methodology: Roselle seeds were germinated in copper sulphate solutions, the seeds were inoculated with bacterial suspensions of two strains of Pseudomonas M40 and M67, the germination percentages were determined and vigour tests were also carried out. Results: The effect of copper sulphate on germination was significant in the interaction between main factors, but no significance was found in the effect of the strains and their interaction with the varieties. In the vigour tests, significance was detected between factors of stem dry weight, root and stem length, and strains. Implications: The tolerance of the seeds to copper sulphate, to know if it has an effect of promoting germination or, on the contrary, inhibits it. Conclusion: copper sulphate has the effect of promoting the germination of roselle seeds. Jamaica has a potential effect of phytoremediation of metals in combination with the inoculation of rhizobacteria that promote plant growth.

Keywords

• fitorremediación
• inoculación de bacterias
• metales pesados
• pruebas de vigor
• porcentaje de germinación
• pseudomonas.