A smartphone-based fluorospectrophotometer and ratiometric fluorescence nanoprobe for on-site quantitation of pesticide residue

Meng Xiao; Ningxia Xu; Aitong He; Zipei Yu; Bo Chen; Baohui Jin; Lelun Jiang; Changqing Yi

iScience (Apr 2023)

A smartphone-based fluorospectrophotometer and ratiometric fluorescence nanoprobe for on-site quantitation of pesticide residue

Meng Xiao,
Ningxia Xu,
Aitong He,
Zipei Yu,
Bo Chen,
Baohui Jin,
Lelun Jiang,
Changqing Yi

Affiliations

Meng Xiao: Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, China; Department of Clinical Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510000, China
Ningxia Xu: Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, China
Aitong He: Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, China
Zipei Yu: Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, China
Bo Chen: Food Inspection and Quarantine Center, Shenzhen Customs, Shenzhen 518033, China
Baohui Jin: Food Inspection and Quarantine Center, Shenzhen Customs, Shenzhen 518033, China; Corresponding author
Lelun Jiang: Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, China; Corresponding author
Changqing Yi: Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, China; Research Institute of Sun Yat-Sen University in Shenzhen, Shenzhen 518057, China; Corresponding author

Journal volume & issue: Vol. 26, no. 4
p. 106553

Abstract

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Summary: Cost-effective and user-friendly quantitation at points-of-need plays an important role in food safety inspection, environmental monitoring, and biomedical analysis. This study reports a stand-alone smartphone-based fluorospectrophotometer (the SBS) installed with a custom-designed application (the SBS-App) for on-site quantitation of pesticide using a ratiometric sensing scheme. The SBS can collect fluorescence emission spectra in the wavelength range of 380-760 nm within 5 s. A ratiometric fluorescence probe is facilely prepared by directly mixing the blue-emissive carbon nanodots (the Fe3+-specific fluorometric indicator) and red-emissive quantum dots (the internal standard) at a ratio of 11.6 (w/w). Based on the acetylcholinesterase/choline oxidase dual enzyme-mediated cascade catalytic reactions of Fe2+/Fe3+ transformation, a ratiometric fluorescence sensing scheme is developed. The practicability of the SBS is validated by on-site quantitation of chlorpyrifos in apple and cabbage with a comparable accuracy to the GC-MS method, offering a scalable solution to establish a cost-effective surveillance system for pesticide pollution.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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