Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq

Inga Rødahl; James Gotley; Stacey B. Andersen; Meihua Yu; Ahmed M. Mehdi; Angelika N. Christ; Emma E. Hamilton-Williams; Ian H. Frazer; Samuel W. Lukowski; Janin Chandra

STAR Protocols (Dec 2021)

Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq

Inga Rødahl,
James Gotley,
Stacey B. Andersen,
Meihua Yu,
Ahmed M. Mehdi,
Angelika N. Christ,
Emma E. Hamilton-Williams,
Ian H. Frazer,
Samuel W. Lukowski,
Janin Chandra

Affiliations

Inga Rødahl: Center for Infectious Medicine, Department of Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden; The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia
James Gotley: The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia
Stacey B. Andersen: The Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia; Corresponding author
Meihua Yu: The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia
Ahmed M. Mehdi: The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia
Angelika N. Christ: The Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia
Emma E. Hamilton-Williams: The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia
Ian H. Frazer: The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia
Samuel W. Lukowski: The Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia; Corresponding author
Janin Chandra: The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100842

Abstract

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Summary: Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species.For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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