Molecular Characterization and Comparative Sequence Analysis of Defense-Related Gene, <em>Oryza rufipogon</em> Receptor-Like Protein Kinase 1

Abstract

Many of the plant leucine rich repeat receptor-like kinases (LRR-RLKs) have been found to regulate signaling during plant defense processes. In this study, we selected and sequenced an <em>LRR-RLK </em>gene, designated as <em>Oryza rufipogon receptor-like protein kinase 1</em> (<em>OrufRPK1</em>), located within yield QTL <em>yld1.1 </em>from the wild rice <em>Oryza rufipogon</em> (accession IRGC105491). A 2055 bp coding region and two exons were identified. Southern blotting determined <em>OrufRPK1 </em>to be a single copy gene. Sequence comparison with cultivated rice orthologs (<em>OsI219RPK1, </em><em>OsI9311RPK1 </em>and<em> OsJNipponRPK1, </em>respectively derived from <em>O. sativa</em> ssp. <em>indica</em> cv. MR219,<em> </em><em>O. sativa</em> ssp. <em>indica</em> cv. 9311 and <em>O. sativa</em> ssp. <em>japonica</em> cv. Nipponbare) revealed the presence of 12 single nucleotide polymorphisms (SNPs) with five non-synonymous substitutions, and 23 insertion/deletion sites. The biological role of the <em>OrufRPK1</em> as a defense related LRR-RLK is proposed on the basis of cDNA sequence characterization, domain subfamily classification, structural prediction of extra cellular domains, cluster analysis and comparative gene expression.

Keywords

• &lt;em&gt;Oryza rufipogon&lt;/em&gt;
• RPK1
• LRR domain
• kinase domain
• disease resistance